Molecular Profiling of Neurons Based on Connectivity
Here, we describe the development and application of Retro-TRAP for projection-specific molecular profiling of diverse cell types across the mammalian brain.
Rapid Molecular Profiling of Neurons Based on Pattern of Projection with Retro-TRAP
This paper describes the protocols for carrying out Retro-TRAP studies, using generalized and cell type-specific approaches.
Plasmids publicly available from Addgene for Retro-TRAP studies.
Cre-dependent, ‘Introverted’ NBL10 construct for cell type-specific Retro-TRAP studies, with 5’ tags for multiplexed profiling of other local, defined cell types.
Cre-dependent NBL10 construct for cell type-specific Retro-TRAP studies.
Viral TRAP (vTRAP)
Rapid Molecular Profiling of Defined Cell Types Using Viral TRAP
This paper describes the development of vTRAP, which uses an adeno-associated viral vector for cell type-specific molecular profiling of diverse cell types across the mammalian brain for which there exists a Cre-driver line.
Constructs for conditional recombinase expression
Plasmid publicly available from Addgene for intersectional genetics studies.
AAV construct for FLP-dependent Cre expression.