Molecular Tools

Retro-TRAP

Molecular Profiling of Neurons Based on Connectivity

Here, we describe the development and application of Retro-TRAP for projection-specific molecular profiling of diverse cell types across the mammalian brain.

Rapid Molecular Profiling of Neurons Based on Pattern of Projection with Retro-TRAP

This paper describes the protocols for carrying out Retro-TRAP studies, using generalized and cell type-specific approaches.

Retro-TRAP constructs

Plasmids publicly available from Addgene for Retro-TRAP studies.

PLASMID

Cre-dependent, ‘Introverted’ NBL10 construct for cell type-specific Retro-TRAP studies, with 5’ tags for multiplexed profiling of other local, defined cell types.

PLASMID

Cre-dependent NBL10 construct for cell type-specific Retro-TRAP studies.

Viral TRAP (vTRAP)

Rapid Molecular Profiling of Defined Cell Types Using Viral TRAP

This paper describes the development of vTRAP, which uses an adeno-associated viral vector for cell type-specific molecular profiling of diverse cell types across the mammalian brain for which there exists a Cre-driver line.

vTRAP Construct

Plasmids publicly available from Addgene for vTRAP studies.

PLASMID

Cre-dependent GFPL10 construct for vTRAP studies.

PLASMID

Cre-dependent, ‘Introverted’ GFPL10 construct for vTRAP studies.

Intersectional Genetics

Constructs for conditional recombinase expression

Plasmid publicly available from Addgene for intersectional genetics studies.

PLASMID

AAV construct for FLP-dependent Cre expression.

Lab Protocols

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